Laboratory-based Short Course (1)
Course Duration: 4 days
Maximum Class Size: 10
Overview
This specialised course focuses on the application of nucleic acid-based techniques,
in particular, those used in the detection of foodborne pathogens. Participants
should have a good grounding in the theory of molecular biology methods and
ideally, have had experience working in a lab employing these methods. A particular
interest in the food microbiology field is also an advantage.
Topics:
(i) Theory and methodology. A brief overview.
Course Units: 3 hours.(ii) Standard analysis of food samples. Issues covered include:
(a) nature of the sample and its effect on the application of molecular methods
(b) steps during standard analysis of food samples at which molecular methods can be applied
(c) appropriate sample preparation for subsequent steps
(d) interpretation of results obtained and possible accompanying tests for further verification.Course Units: 6 hours.
(iii) Protocols. Specific protocols for the use of molecular tools in detection of foodborne pathogens will be presented, discussed and applied. Participants will gain hands-on experience, using the protocols in the lab setting.
Course Units: 15 hours.